Key features and details | |
Cat. No. | MABL-1070 |
Name | Anti-CD68 mAbs |
Clone No. | AFD- FA-11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine CD68, known also as macrosialin (MS). CD68 is a 110 kDa, heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the lysosomal/endosomal-associated membrane glycoproteins (LAMP). CD68 is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several acute myeloid leukemia studies. |
Alternative Name | Macrosialin; FA/11; FA11 antigen |
UniProt | P31996 |
Immunogen | This antibody was raised by immunising rats (AO x Lou strain) with murine macrosialin (MS). |
Application Notes | When it was first generated, this antibody was used in binding assays, immunoprecipitation (IP) and immunofluorescence (IF) to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes (Smith et al., 1987). Subsequently, this antibody has been utilised in numerous FACS, IP, IHC, ICC/IF, WB applications, for instance, to delineate the roles of mouse and human CD68 as macrophage receptors for oxidised low density lipoprotein (Ramprasad et al., 1996), to indicate that mice unresponsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) are unexpectedly resistant to cutaneous Leishmania major infection (Scott et al., 2000), to characterise the macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice (Lang et al., 2000), and to examine the role of Foxp3(+) Tregs in Alzheimer's disease (Baruch et al., 2015). |
Antibody First Published | Smith et al. Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. J Cell Sci. 1987 Feb;87 ( Pt 1):113-9. PMID:3312248 |
Note on publication | Describes the original generation of this antibody and its subsequent applications in binding assays, immunoprecipitation and immunofluorescence to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
