Anti-CD66e mAbs

Cat. No.

MABL-1069

Name

Anti-CD66e mAbs

Clone No.

AFD- CB-30

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for CD66e.

Alternative Name

CEA; CEAM5; Meconium antigen 100;   Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion   molecule 5

UniProt

P06731

Immunogen

The original antibody was raised by   immunizing a BALB/c mouse with CD66e.

Application Notes

The original version of this antibody was used for flow cytometry on human colorectal cancer cell lines (Ray et al.,   2012; PMID:22606209). Furthermore while studying human colorectal carcinomas,   this antibody was used for immunohistochemistry on colon tumor sections. This   antibody was also used for western blot on normal murine colon cell lysates   and transformed colon cells lysates (McNeil et al., 2016; PMID:27750367).

Antibody First Published

Ray et al. Establishment of human ultra-low   passage colorectal cancer cell lines using spheroids from fresh surgical   specimens suitable for in vitro and in vivo studies J Cancer. 2012:3:196-206.   PMID:22606209

Note on publication

The original paper studied the establishment of tumor initiating colon cancer ultra- low passage cell lines by a combination of gentle mechanical, enzymatic dissociation, spheroid formation, and followed by two generation xenografts from fresh tumors obtained at time of operation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.