Key features and details | |
Cat. No. | MABL-1031 |
Name | Anti-CD50 mAbs |
Clone No. | AFD- 186 2G9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD50. |
Alternative Name | ICAM3; CDw50; ICAM-R; ICAM-3 |
UniProt | P32942 |
Immunogen | The original antibody was raised by immunizing a mouse with CD50. |
Application Notes | The original version of this antibody was used to immunophenotype mast cells through flow cytometry, while studying the cell surface markers of human skin mast cells. (Ghannadan et al., 1998; PMID:9764855). This antibody was used to block CD50, to determine the effect of the CD50 protein on PMN attachment (Sud'ina et al., 2001; PMID:11672437). This antibody was able to stain CD50 in a immunohistochemistry experiment on psoriasis tissue. This experiment was performed to study the mechanism of action of methotrexate (Yazici et al., 2005; PMID:16215763). |
Antibody First Published | Ghannadan et al. Phenotypic characterization of human skin mast cells by combined staining with toluidine blue and CD antibodies J Invest Dermatol. 1998 Oct;111(4):689-95.PMID:9764855 |
Note on publication | The original paper esthablished the cell surface marker profile of human skin MC using monoclonal antibodies (MoAb) against defined CD antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
