Key features and details | |
Cat. No. | MABL-1030 |
Name | Anti-CD50 mAbs |
Clone No. | AFD- 101-1D2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, IP, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes CD50 domain 1. |
Alternative Name | ICAM3; CDw50; ICAM-R; ICAM-3 |
UniProt | P32942 |
Immunogen | The original antibody was raised by immunizing mice with a mixed leukocyte culture. |
Application Notes | The original version of this antibody was used for immunofluorescence on T cells, B cells and non lymphoic cell lines (Juan et al., 1993; PMID:8325327), on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), and on peripheral blood stimulated or unstimulated cells . Furthermore this antibody was used for flow cytometry on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), on peripheral blood stimulated or unstimulated cells (Vilella et al., 1990; PMID:1710077), and on T cells (Vilardell et al., 1998; PMID: 9672149). This antibody was also used for immunoprecipitation on PMBCs to determine whether CD50 and ICAM-3 are the same protein (Juan et al., 1993; PMID:8325327), and on peripheral blood stimulated or unstimulated cells to prove that 101–1D2 and 140–11 recognize the same molecule (Vilella et al., 1990; PMID:1710077). This antibody was used for immunohistochemistry on human neoplasms to study the expression of CD50 (Terol et al., 1996; PMID: 8946680). |
Antibody First Published | Vilella et al. Involvement of the CDw50 molecule in allorecognition Tissue Antigens. 1990 Nov;36(5):203-10. PMID:1710077 |
Note on publication | The original paper shows that 101–1D2 and 140–11 recognize a different epitope of the same molecule. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
