Key features and details | |
Cat. No. | MABL-960 |
Name | Anti-CD43 mAbs |
Clone No. | AFD- OX-56 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB |
Species Reactivity | Rat |
Basic Information | |
Specificity | OX-56 binds specifically to CD43 and binding is not affected by CD43 glycosylation. OX-56 reacts with most thymocytes and T lymphocytes in the lymph node cell population. This antibody also binds to pre-B cells and can detect CD43 at low levels on B cells. CD43 plays a role in the physicochemical properties of the T-cell surface and in lectin binding. CD43 is a major cell-surface sialoglycoprotein - also found on neutrophils, bone marrow stem cells, B-lineage cells after activation of thymocytes and T lymphocytes but is not expressed on resting B lymphocytes. The protein presents carbohydrate ligands to selectins and Is a counter-receptor for SN/Siglec-1. During T-cell activation it is actively removed from the T- cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. |
Alternative Name | Leukosialin; OX56; MRC OX-56; MRC OX56; sialophorin |
UniProt | P13838 |
Immunogen | OX-56 was prepared by immunizing BALB/c mice with activated rat T lymphocytes. |
Application Notes | OX-56 has been used in the purification of rat leukosialin by immunoaffinty chromatography (Willis et al. 1987) as well as in WB. |
Antibody First Published | Cyster et al The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation. EMBO J. 1991 Apr;10(4):893-902. PMID:1706994 |
Note on publication | Describes the use of OX-56 in the Epitope mapping of leukosialin |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
