Cat. No.
MABL-2141
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb13
From
Recombinant Antibody
Specificity
This antibody binds human insulin. Insulin is a peptide hormone produced by the beta cells of the pancreatic islets. It reduces blood glucose concentration by promoting its absorption from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes mellitus, a condition of high blood sugar level (hyperglycemia).
Alternative Names
INS; mab13 S58R; 13G12
UniProt
P01308
Immunogen
The original antibody was generated from the peripheral B cells obtained from an insulin- dependent (type I) diabetes mellitus (IDDM) patient treated with human recombinant insulin, by EBV transformation and somatic cell hybridization techniques.
Application Notes
This antibody is an affinity matured version of mab13 created by a point mutation in the variable heavy region S58R. The affinity of the IgG1 version of this antibody for insulin was found to be kd=59nM (PMID: 7995943). The binding specificity of the original IgG1 antibody for human insulin was determined using ELISA (PMID: 2159034).
Antibody First Published
Casali et al. Frequency of B cells committed to the production of antibodies to insulin in newly diagnosed patients with insulin-dependent diabetes mellitus and generation of high affinity human monoclonal IgG to insulin. J Immunol. 1990 May 15;144(10):3741-7. PMID:2159034
Note on publication
Describes the generation of high affinity anti-insulin antibodies from B cells of patients suffering from insulin-dependent (type I) diabetes mellitus (IDDM).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
