Key features and details | |
Cat. No. | MABL-928 |
Name | Anti-CD4 mAbs |
Clone No. | AFD- GK1.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for CD4. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. |
Alternative Name | T-cell surface glycoprotein CD4; Cd4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3 |
UniProt | P06332 |
Immunogen | The antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll- Hypaque-purified cells from the cloned CTL line 243/2.5 |
Application Notes | The effect of this antibody on the PFC response was measured by treating spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170).Immunofluorescence was preformed preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). An flow cytometry experiment was preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). The effect of this antibody on antigen-specific release of IL2 by mouse T cell hydridomas (Wilde et al, 1983; pmid:6195255). ELISA was preformed with this antibody on calf fetal serum (Wilde et al, 1983; pmid:6195255). The effect of the antibody on antigen-specific binding by T cell hybridoma AODH-3.4 was determined (Wilde et al, 1983; pmid:6195255). |
Antibody First Published | Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule J Immunol. 1983 Nov;131(5):2445-51.PMID:6415170 |
Note on publication | In the paper the antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll-Hypaque-purified cells from the cloned CTL line 243/2.5. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
