Anti-CD4 mAbs

Cat. No.

MABL-927

Name

Anti-CD4 mAbs

Clone No.

AFD- DB81

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralising, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD4, and recognises an epitope in the vicinity of a 13- amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.

Alternative Name

Lu/T4; T-cell surface glycoprotein CD4;   T-cell surface antigen T4/Leu-3

UniProt

P01730

Immunogen

This antibody was raised by immunising   BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).

Application Notes

This antibody preferentially binds to a   complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance   analysis has shown that the binding affinity of this antibody to the CD4-   gp120 complex is almost one order of magnitude higher than its binding to CD4   alone (Burastero et al, 2011), while ELISA analysis has confirmed that the   epitope recognised by this antibody does not involve gp120 (Burastero et al,   2011). This antibody, through a post-binding mechanism, inhibits the cell   fusion and replication of a range of HIV-1 strains (Burastero et al, 2011).   It does not exert inhibitory effects on T-cell activation in vitro, a useful   property for clinical use.

Antibody First Published

Burastero et al. Broad-Spectrum Inhibition of HIV-1 by a Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 PLoS One. 2011; 6(7): e22081. PMID:21818294

Note on publication

Describes the original generation of this   antibody, and characterisation of its binding specificity.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.