Key features and details | |
Cat. No. | MABL-922 |
Name | Anti-CD4 mAbs |
Clone No. | AFD- OX-68 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | binding assay, IP, SPR, WB, FC |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69. |
Alternative Name | Leu3/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu- 3; W3/25 antigen |
UniProt | P05540 |
Immunogen | This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously. |
Application Notes | This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4. |
Antibody First Published | Simon et al. Role of CD4 epitopes outside the gp120-binding site during entry of human immunodeficiency virus type 1. J Virol. 1997 Feb;71(2):1476-84. PMID:8995673 |
Note on publication | Describes the original use of this antibody to elucidate the role of CD4 epitopes outside the gp120-binding site during HIV-1 entry. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
