Key features and details | |
Cat. No. | MABL-921 |
Name | Anti-CD3e mAbs |
Clone No. | AFD- Visilizumab (HuM291) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunesuppression, therapeutic, FC |
Species Reactivity | chimpanzee, Human |
Basic Information | |
Specificity | This antibody recognises the human CD3 epsilon. This antibody also competes with OKT3 (Muromonab) for binding site on the human T cells suggesting that they bind overlapping or closely spaced epitopes. |
Alternative Name | T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε |
UniProt | P07766 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with activated human T cells as immunogens. |
Application Notes | This antibody is derived from its a murine parent antibody called M291. HuM291 was significantly less mitogenic to T cells in vitro as compared to OKT3 and induced the release of much lower levels of the cytokines tumor necrosis factor-α, interferon-γ, and interleukin-10. HuM291 still retained the ability to modulate the CD3 complex and inhibit the mixed lymphocyte reaction. This antibody competes with OKT3 for binding to human T cells, which was detected using flow cytometry (PMID: 10480417). In vitro studies using chimpanzee peripheral blood mononuclear cells (PBMCs), HuM291 was reported to be less mitogenic than OKT3 and effectively depleted peripheral T cells without eliciting clinical signs of cytokine release syndrome, and recovered T cells were functionally normal (PMID: 10480415). |
Antibody First Published | Cole et al. HuM291, a humanized anti-CD3 antibody, is immunosuppressive to T cells while exhibiting reduced mitogenicity in vitro. Transplantation. (1999); 68(4):563-71.PMID:10480417 |
Note on publication | Describes the generation, characterization and comparison o f this antibody to another anti-CD3e antibody OKT3. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
