Cat. No.
MABL-1207
Application
RIA, Western blot, ELISA
Isotype
Engineer antibody
Species Reactivity
Vibrio cholerae
Clone No.
TE33
From
Recombinant Antibody
Specificity
This antibody binds the CTP3 loop of the B subunit of the cholera toxin. This antibody is not capable of binding cholera toxin in its native form or its wild type form. TE33 specifically recognizes a loop comprising the sequence 'VPGSQHID' and a beta-turn formed by the sequence 'VPGS'. The CTP3 peptide is involved in saccharide binding to the receptor on the epithelial cells.
Alternative Names
ctxB; toxB; VC_1456; Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid
UniProt
P01556
Immunogen
The original antibody was generated by immunizing mice with CTP3 which is a conserved loop comprising amino acid residues 50-64 on the surface of the B subunit. CTP3 is involved in saccharide binding to the receptor on epithelial cells.
Application Notes
The original IgG1 antibody which was raised against the CTP3 peptide also cross reacts with cholera toxin in a solid phase ELISA. The binding of the original IgG1 antibody to cholera toxin and H- LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli was done using solid-phase RIA and western blot (PMID: 2450576). In solution, the original antibody has a thousandfold lower affinity for the cholera toxin as compared to that for CTP3 (PMID: 1379072). The binding of the original antibody to both CTP3 peptide and cholera toxin was assayed by ELISA, solid-phase radioimmunoassay and immunoblot assay. The antibody was incapable of binding cholera toxin in its native form when tested in a solution- phase assay system (PMID: 1993848).
Antibody First Published
Anglister et al. NMR study of the complexes between a synthetic peptide derived from the B subunit of cholera toxin and three monoclonal antibodies against it. Biochemistry. 1988 Jan 26;27(2):717-24. PMID:2450576
Note on publication
The contact interaction between a synthetic peptide CTP3 (residues 50-64 of the B subunit of cholera toxin) suggested as a possible epitope for synthetic vaccine against cholera and three different anti-peptide monoclonal antibodies were studied by nuclear magnetic resonance (NMR).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
