Cat. No.
MABL-1206
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Vibrio cholerae
Clone No.
A9 (LCTA9)
From
Recombinant Antibody
Specificity
This antibody binds the B subunit of the cholera toxin.
Alternative Names
ctxB; toxB; VC_1456; Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid
UniProt
P01556
Immunogen
The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas.
Application Notes
The binding specificity of this antibody towards cholera toxin was checked using ELISA (PMID:17165813). SPR was used to determine the dissociation constant (Kd) of VHH A9; the Kd was determined to be 77 ± 5 nM (PMID: 23385744). It was reported that two point mutations (N27D and R71I) help stabilize A9 wild-type form which has a relatively low melting point (PMID: 31342597).
Antibody First Published
Goldman et al. Facile Generation of Heat Stable Antiviral and Antitoxin Single Domain Antibodies from a Semi-synthetic Llama Library. Anal Chem. 2006 Dec 15; 78(24): 8245–8255. PMID:17165813
Note on publication
A semisynthetic llama library was constructed from non-immune llamas and binders specific for live vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococcal enterotoxin B were selected.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
