Cat. No.
MABL-1182
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5D1
From
Recombinant Antibody
Specificity
This antibody binds human major centromere autoantigen B (CENP-B). It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Alternative Names
CENPB; Centromere protein B; Major centromere autoantigen B
UniProt
P07199
Immunogen
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
Application Notes
The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human CENP-B in a western blot assay (PMID: 18062792).
Antibody First Published
Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens. Clin Exp Immunol. 2008 Feb; 151(2): 367–377. PMID:18062792
Note on publication
Describes the generation of chicked antibodies against various human autoantigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
