Cat. No.
MABL-1181
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Streptococcus downei, Streptococcus sobrinus
Clone No.
OMVU10
From
Recombinant Antibody
Specificity
This antibody reacts with the purified cell-wall antigen B of Streptococcus sobrinus, which is an anaerobic, gram-positive, catalase-negative, non-motile bacteria. it also cross reacts with Streptococcus downei. Streptococcus sobrinus is a pathogenic bacteria in humans which enhances the formation of caries within teeth. S. sobrinus is also affiliated with early childhood caries, which are responsible for the majority of dental abscesses and toothaches in children.
Alternative Names
Group B cell wall antigen; polysaccharide antigen of group B
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with bacterial cell suspension of strain HG459 of Streptococcus sobrinus.
Application Notes
This antibody can be used for the identification of different strains of Streptococcus sorbinus using ELISA and immunofluorescence assay. This antibody was not found to cross react with strains other than S. sobrinus (PMID: 3323224). This antibody was used for the enumeration of Streptococci from dental plaque or saliva with the colony immunoblot technique. This antibody could not directly bind S. sobrinus in dental plaque samples, thus the samples were cultured prior to immunological detection and identification (PMID: 2254421).
Antibody First Published
Soet et al. Identification of Streptococcus sobrinus with monoclonal antibodies. Antonie Van Leeuwenhoek. 1990 Nov; 58(4):219-25. PMID:3323224
Note on publication
Describes the generation of two antibodies against Streptococcus sorbinus.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
