Cat. No.
MABL-1171
Application
ICC, RIA, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12E 7
From
Recombinant Antibody
Specificity
This antibody binds human CD99. CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. It plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane.
Alternative Names
CD99 antigen; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; 12E7; MIC2; MIC2X; MIC2Y
UniProt
P14209
Immunogen
The original antibody was generated by immunizing BALB/c mice intraperitoneally with glutaraldehyde-fixed human acute lymphocytic leukemia (ALL) cells obtained from peripheral blood of a child.
Application Notes
The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewing's sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).
Antibody First Published
Levy et al. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6552-6. PMID:316541
Note on publication
Describes the use of this antibody in identification and characterization of human thymus leukemia antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
