Cat. No.
MABL-1140
Application
IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Pig, Human
Clone No.
3D1
From
Recombinant Antibody
Specificity
This antibody binds human B7-2 (CD86).
Alternative Names
B7-2; B7.2; B70; BU63; CD28LG2; FUN-1; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; CTLA-4 counter-receptor B7.2; CTLA4L; CTLA-4L
UniProt
P42081
Immunogen
The original antibody was generated by immunizing mice with human B7-2.
Application Notes
The original human IgG1, 3D1 antibody binds human B7-2 with an affinity of approximately Kd= 1nM and can be used to identify human B7-2 in an ELISA. This antibody was capable of immunoprecipitating B7-2 exrpressing porcine microvascular endothelial cells (PMID： 8757955). This antibody was capable of identifying CHO cells transfected with B7-2 in a flow cytometric assay.
Antibody First Published
Davis et al. Primary porcine endothelial cells express membrane-bound B7-2 (CD86) and a soluble factor that co-stimulate cyclosporin A-resistant and CD28-dependent human T cell proliferation. Int Immunol. (1996); 8(7)：1099-111. PMID：8757955
Note on publication
Describes the use of this antibody for detection of B7-2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
