Cat. No.
MABL-1119
Application
immunotoxin, IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Macaca nemestrina, Human
Clone No.
G10-1
From
Recombinant Antibody
Specificity
This antibody is specific for human CD8, and binds human CD8+ T cells. It also cross-reacts with non-human primate CD8, including that of Macaca nemestrina (Southern pig-tailed macaque).
Alternative Names
cluster of differentiation 8; G10.1; Tp32
UniProt
Immunogen
Human CD8.
Application Notes
This antibody has been used to stain cells expressing CD8 by indirect immunofluorescence (Uckun et al, 1989) and in immunohistochemical analysis of aortic and iliac artery tissue from nonhuman primates (Masuda & Ross, 1989). It has also been employed in immunoprecipitation and gel electrophoresis experiments to investigate cell surface CD8 complexes (Ledbetter et al, 1985). A FITC-conjugated version of this antibody has been used to identify T cell populations in flow cytometry experiments (Chen et al, 2003). When conjugated to the ribosome-inhibitory protein ricin, this antibody acts as a potent CD8+ T cell-directed immunotoxin (Uckun et al, 1989).
Antibody First Published
Ledbetter et al. Covalent association between human thymus leukaemia-like antigens and CD8(Tp32) molecules. J Immunol. 1985 Jun;134(6):4250-4 PMID:3921621
Note on publication
Describes the use of this antibody in immunoprecipitation and gel electrophoresis experiments
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
