Cat. No.
MABL-1110
Application
FC, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Koala, Human
Clone No.
AT107-2
From
Recombinant Antibody
Specificity
This antibody specific for the intracellular domain of human CD79b. CD79b is required in tandem with CD79A for initiation of the signal transduction cascade induced by the B-cell antigen receptor complex which leads to internalization of the complex, trafficking to late endosomes and antigen presentation.
Alternative Names
B-cell antigen receptor complex-associated protein beta chain; CD79B; B- cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein
UniProt
Immunogen
The rat version of this antibody was raised by immunizing a mouse with the intracellular domain of CD79B.
Application Notes
To study chronic lymphocytic leukemia, immunohistochemistry was performed on tissues from patients with CLL or RT using the rat version of this antibody (Allan et al, 2019). Tissue from non-Hodgkin lymphoma and chronic lymphocytic leukemia patients was stained for immunohistochemistry using the rat version of this antibody to create an antibody–drug conjugate consisting of a humanized anti- CD79b antibody that is conjugated to monomethylauristatin E. This construct is supposed to help the patients with these forms of cancer (Dornan et al, 2009; pmid:19633198). To investigate anti CD79b clone DCDS0780A as a possible therapeutic agent against B-NHL, immunohistochemistry was performed on tissues from B-NHL patients that had relapsed after or failed to respond to at least one prior treatment regimen using the rat version of this antibody (Herrera et al, 2022; pmid:34980599). To determine the expression of CD79b in tumor cells immunohistochemistry was performed on tissues from R/R DLBCL patients using the rat version of this antibody (Sehn et al, 2019; pmid:31693429). To determine the lymphoma isotype of dogs, flow cytometry was performed on lymph node cells using the rat version of this antibody (Thalheim et al, 2013; pmid:24112291). To understand immune activity in koalas, flow cytometry was performed on peripheral blood leukocytes of koalas using the rat version of this antibody (Lau et al, 2012; pmid:22551982). To characterize a CD4+ CD25 high FOXP3 high regulatory T-cell population in dogs, flow cytometry was used on PMBCs using the rat version of this antibody (Pinheiro et al, 2011; pmid:20880379).
Antibody First Published
Dornan et al. Therapeutic potential of an anti-CD79b antibody-drug conjugate, anti-CD79b-vc-MMAE, for the treatment of non-Hodgkin lymphoma Blood. 2009 Sep 24;114(13):2721-9. PMID:19633198
Note on publication
An antibody-drug conjugate (ADC) consisting of a humanized anti-CD79b antibody that is conjugated to monomethylauristatin E was created.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
