Cat. No.
MABL-1089
Application
Cristallography, in vitro, in vivo
Isotype
Engineer antibody
Species Reactivity
Monkey, Human
Clone No.
ch128.1
From
Recombinant Antibody
Specificity
The antibody binds the apical domain of hTfR1.
Alternative Names
TFRC; TfR1; TR; TfR; hTfR1; Transferrin receptor protein 1; p90; 128.1; anti-hTfR IgG3
UniProt
P02786
Immunogen
The antibody was constructed by substituting the variable regions of anti-dansyl IgG3 with those of the murine IgG1 anti-human TfR monoclonal Ab (mAb) 128.1. Ab128.1 was generated by immunizing mice with the external domain of the human transferrin (Tf) receptor.
Application Notes
The IgG3 format of the antibody cross-linked with a secondary antibody inhibits the growth and induces apoptosis in ARH-77 cells. The IgG3 format of the antibody cross-linked with a secondary Ab induces intracellular degradation of the TfR (Ng et al., 2006; PMID: 16804109). The IgG3 format of the antibody was able to block the entry of MLV pseudotyped with the envelope glycoproteins of JUNV, GTOV, CHAV, SABV, and MACV in CHO-K1 cells. The 50% inhibitory concentration (IC50) of the IgG3 format of the antibody for these pseudoviruses was measured in HEK293T cells. The antibody showed subnanomolar IC50 for all NW hemorrhagic fever arenaviruses. The IgG3 format of the antibody could antagonize replication-competent JUNV. Surface plasmon resonance assays confirmed that the IgG3 format of the antibody has a high affinity for hTfR1 (Kd = 5.7 nM). The IgG3 format of the antibody detected CD71 in different kind of cells (HEK 293T, sMAGI, Vero) by flow cytometry. The antibody was able to block JUNV pseudovirus entry in sMAGI and Vero cells (Helguera et al., 2012; PMID: 22278244). The IgG1 format of the antibody could inhibit JUNV infection in vitro (EC90 = 0.73 nM). The IgG1 format of the antibody protects hTfR1-expressing transgenic mice against lethal NWM challenge. The crystal structure of the Fab fragment of the antibody was determined (Hickerson et al., 2022; PMID: 35091550).
Antibody First Published
White et al. Combinations of anti-transferrin receptor monoclonal antibodie inhibit human tumor cell growth in vitro and in vivo: evidence for synergistic antiproliferative effects Cancer Res. 1990 Oct 1;50(19):6295-301. PMID:2400993
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
