Cat. No.
MABL-1072
Application
FACS, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Y1/82A
From
Recombinant Antibody
Specificity
This antibody recognises human CD68 antigen. The Y1/82A antibody recognises an epitope distinct from those of antibody clones Y2/131, EBM11, Ki-M6 and KP1. Y1/82A is reportedly more specific for monocytes and macrophages than KP1. Its antigen, CD68 is a membrane glycoprotein highly expressed in monocytes and macrophages. It is involved in phagocytosis, lysosomal metabolism and interactions with pathogens and other cells.
Alternative Names
Macrosialin; Gp110; DKFZp686M18236; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1; CD68 molecule; CD68 antigen; Macrophage Antigen CD68
UniProt
P34810
Immunogen
This antibody was raised by immunising BALB/c mouse with phytohaemagglutinin stimulated blasts.
Application Notes
This antibody was demonstrated to react with peripheral blood and bone marrow monocytes and resident macrophages in all tissues tested and was also able to characterise certain leukemias (Davey et al., 1988). Furthermore, it was proposed that Y1/82A may be useful in the diagnosis of monocytic leukaemias and histiocytic neoplasms as well as in the detection of macrophages in various diseases (Davey et al., 1988). For instance, one group utilised it during flow cytometric description of the expression of various surface markers on 1A1 and C06 cells in a study on microglia during HIV infection (Garcia-Mesa et al., 2017). Similarly, another group used this antibody to characterise vesicle cells during Varicella-Zoster Virus infection (Carpenter et al., 2011).
Antibody First Published
Davey et al. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988 Jul;41(7):753-8. PMID:2457604
Note on publication
This article describes generation and characterisation of Y1/82A antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
