Cat. No.
MABL-1064
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
NK-1-C3
From
Recombinant Antibody
Specificity
This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades.
Alternative Names
Granulophysin; LAMP-3; Tetraspanin-30; Tspan-30; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; CD63 antigen; NK1/C3; NKI/C3; NK-1.C3; NK1C3
UniProt
P08962
Immunogen
This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
Application Notes
The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016).
Antibody First Published
Vennegoor et al. Biochemical characterization and cellular localization of a formalin-resistant melanoma-associated antigen reacting with monoclonal antibody NKI/C-3. Int J Cancer. 1985 Mar 15;35(3)11:45287-95. PMID:3882581
Note on publication
This article describes the generation and characterization of this anntibody (NKI/C-3).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
