Anti-CD56 mAbs [NKINBL1](MABL-1044)

MABL-1044

Depletion, WB, FC, IHC

Engineer antibody

Human

NKINBL1

Recombinant Antibody

This antibody is specific for human CD56. CD56, is a homophilic binding glycoprotein expressed on the surface of neurons, glia and skeletal muscle. Although CD56 is often considered a marker of neural lineage commitment due to its discovery site, CD56 expression is also found in, among others, the hematopoietic system.

NKI-NBL-1; Neural cell adhesion molecule 1; NCAM1; N-CAM-1; NCAM-1

P13591

The mouse version of this antibody was raised by immunizing a BALB/c mouse with CHP-212 human neurablastoma cell line.

To quantify CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages, flow cytometry was performed on multiple human cell lines using the mouse version of this antibody (Lee et al, 1999; pmid:10220446). To determine differences in the expression level of selected adhesion molecules to identify suitable tools for the pre- procedural identification of restenosis patients before angioplasty, flow cytometry was performed on PMBCs using the mouse version of this antibody (Rahimi et al, 2003; pmid:12717693). To test the ability of OM-174 to stimulate in vitro the lytic potential of peripheral blood cells from patients with non-metastatic breast tumour, the mouse version of this antibody was used for NK depletion on mononuclear blood cells (Reisser et al, 1999). The mouse version of this antibody was used in whole full blood for flow cytometry to characterize the coreceptor activity and the expression pattern of alternative coreceptors that are used by HIV-1 to enter the cell (Sharron et al, 2000; pmid:10891428). To determine the specificity of the mouse version of the antibody, a western blot was performed on different CD56 isoforms. This antibody showed no specificity for any isoform (Gattenlöhner et al, 2009; pmid:19246644). To study tumour infiltrating dendritic cells, Tissue samples collected at the time of nephrectomy were used for immunohistochemistry using the mouse version of this antibody (Schwaab et al, 1999; pmid:10411089).

Lee et al. Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5215-20. PMID:10220446

A quantitative fluorescence-activated cell sorting assay was used to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites (ABS) on various T cell lines, T cell subsets, peripheral blood dendritic cells (PBDC), and monocyte-derived macrophages by using four-color fluorescence-activated cell sorting analysis on fresh whole blood.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.