Cat. No.
MABL-1036
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HD77
From
Recombinant Antibody
Specificity
This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.
Alternative Names
TSPAN-25; Leukocyte surface antigen CD53; Tetraspanin-25; MOX44; TSPAN25
UniProt
P19397
Immunogen
This antibody was raised by immunising mice with human leukemia cells.
Application Notes
This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001).
Antibody First Published
Mechtersheimer et al. Expression of HLA-A,B,C, beta 2-microglobulin (beta 2m), HLA-DR, -DP, -DQ and of HLA-D-associated invariant chain (Ii) in soft-tissue tumors. Int J Cancer. 1990 Nov 15;46(5):813-23. PMID:2228310
Note on publication
Describes the original use of this antibody, and its use in immunohistochemical analysis of frozen tissue sections.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
