Cat. No.
MABL-1035
Application
Functional Studies, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
161-2 (53/2)
From
Recombinant Antibody
Specificity
This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.
Alternative Names
Tetraspanin-25; Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Tspan-25
UniProt
P19397
Immunogen
This antibody was raised by immunizng mice with stimulated human leukocytes.
Application Notes
This antibody is recommended for the detection and analysis of CD53 via flow cytometry and immunofluorescence. Additionally, one group utilized this antibody for the ligation of the CD53 antigen while researching the potential involvement of tetraspanins (such as CD53) in tumor metastasis (Yunta et al., 2002). Furthermore, CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells, as it is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells.
Antibody First Published
Yunta et al. Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types. Eur J Biochem. 2002 Feb;269(3):1012-21. PMID:11846804
Note on publication
This article describes the use and characterization of the 161-2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
