Cat. No.
MABL-1032
Application
functional assay, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
NKI-M9
From
Recombinant Antibody
Specificity
This antibody is specific for human CD51. CD51 is a receptor for vitronectin. It recognizes the sequence R-G-D in a wide array of ligands.
Alternative Names
Integrin alpha-V; ITGAV; Vitronectin receptor; Vitronectin receptor subunit alpha
UniProt
P06756
Immunogen
The mouse version of this antibody was raised by immunizing a BALB/c mouse with cultured melanoma cells derived from a human melanoma metastasis.
Application Notes
To study MSCs as a promising therapeutic agents for a variety of diseases, flow cytometry was performed on human MSCs using the mouse version of this antibody (Wang et al, 2015; pmid:26148841). To study how Bordetella pertussis enters respiratory epithelial cells, an invasion assay was performed using the mouse version of this antibody on human lung epithelial cell line A549 with Bordetella pertussis (Ishibashi et al, 2001; pmid:11373122). To find surface markers specific for Nestin+ cells, flow cytometry was performed on mouse BM primary cells using the mouse version of this antibody.Furthermore, human foetal bones and HA/TCP grafts were stained using this antibody (Pinho et al, 2013; pmid:23776077). To investigate the developmental pathway for human megakaryocytes, flow cytometry was performed on BM HSPCs from healthy donors using the mouse version of this antibody (Miyawaki et al, 2017; pmid:28336526). To determine if OCs express other membrane receptors for matrix proteins, immunohistochemistry was performed on human joint revision capsule tissue using the mouse version of this antibody (Quinn et al, 1991; pmid:1717412).
Antibody First Published
Quinn et al. Extracellular matrix receptor and platelet antigens on osteoclasts and foreign body giant cells Histochemistry. 1991;96(2):169-76. PMID:1717412
Note on publication
The antigenic phenotype of OCs and foreign body giant cells (FBGCs) was investigated for the presence of several integrin molecules and other largely platelet-associated antigens involved in cell adhesion reactions. Both OCs and FBGCs expressed the alpha-chains of the vitronectin receptor (CD51) and of the VLA-2 (CDw49b) and VLA-4 (CDw49d) molecules as well as their respective beta- chains, gpIIIa (CD61) and CD29.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
