Cat. No.
MABL-1030
Application
inhibition, IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
101-1D2
From
Recombinant Antibody
Specificity
This antibody recognizes CD50 domain 1.
Alternative Names
ICAM3; CDw50; ICAM-R; ICAM-3
UniProt
P32942
Immunogen
The original antibody was raised by immunizing mice with a mixed leukocyte culture.
Application Notes
The original version of this antibody was used for immunofluorescence on T cells, B cells and non lymphoic cell lines (Juan et al., 1993; PMID:8325327), on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), and on peripheral blood stimulated or unstimulated cells . Furthermore this antibody was used for flow cytometry on thymocytes (Martinez-Caceres et al., 1996; PMID:9008304), on peripheral blood stimulated or unstimulated cells (Vilella et al., 1990; PMID:1710077), and on T cells (Vilardell et al., 1998; PMID: 9672149). This antibody was also used for immunoprecipitation on PMBCs to determine whether CD50 and ICAM-3 are the same protein (Juan et al., 1993; PMID:8325327), and on peripheral blood stimulated or unstimulated cells to prove that 101–1D2 and 140–11 recognize the same molecule (Vilella et al., 1990; PMID:1710077). This antibody was used for immunohistochemistry on human neoplasms to study the expression of CD50 (Terol et al., 1996; PMID: 8946680). The ability of this antibody to inhibit cell- mediated lympholysis was tested in an inhibition assay on PMBCs was tested, it showed it was able to inhibit lympholysis (Vilella et al., 1990; PMID:1710077). Finally this antibody was used for western blot on immunoprecipitated proteins. This experiment shows that the CD50 protein coprecipitates with a 56-kD lck protein and a 59-kD fyn PTK (Juan et al., 1994; PMID:7515097).
Antibody First Published
Vilella et al. Involvement of the CDw50 molecule in allorecognition Tissue Antigens. 1990 Nov;36(5):203-10. PMID:1710077
Note on publication
The original paper shows that 101–1D2 and 140–11 recognize a different epitope of the same molecule.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
