Cat. No.
MABL-1021
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Human, Mouse
Clone No.
GoH3
From
Recombinant Antibody
Specificity
This antibody is specific for the mouse, human and dog version of the CD49F protein. CD49F is a receptor for laminin on platelets. Furthermore CD49F is present in oocytes and is involved in sperm-egg fusion.
Alternative Names
Integrin alpha-6; ITGA6; CD49 antigen-like family member F; VLA-6
UniProt
P23229
Immunogen
This antibody was raised by immunizing a BALB/c rat with amino acids 1242-1255 of the C- terminus of the Human c-erbB-2/HER-2/neu protein
Application Notes
To determine the influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells, cryo-sections of human cultured gingiva were stained for immunohistochemistry using the mouse version of this antibody. (Becker et al, 2002; pmid：12013557). To isolate pancreatic islet progenitor cells from foetal mice, a mouse pancreatic cell dispersion was used for flow cytometry using the mouse version of this antibody (Sugiyama et al, 2006; pmid：17190805). To present evidence that HPV binds specifically to laminin 5, cultured keratinocytes infected with VLPs from HPV-11, were stained using the mouse version of this antibody (Culp et al, 2006; pmid：16940506).
Antibody First Published
Becker et al. Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro Eur J Oral Sci. 2002 Apr;110(2)：137-43. PMID：12013557
Note on publication
Monoclonal antibodies against the VLA-6 laminin receptor subunit alpha6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with anti alpha1- antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
