Anti-CD49E mAbs [SAM1](MABL-1019)

MABL-1019

functional assay, FC, IF, IHC

Engineer antibody

Human

SAM1

Recombinant Antibody

This antibody is specific for human CD49E. CD49E is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. CD49E binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.

SAM-1; Integrin Alpha 5

P08648

The mouse version of this antibody was raised by immunizing a BALB/c mouse with U 937 cells.

To determine the potential of human antibodies for use in an endocap-based approach, immunohistochemistry was used on snap-frozen samples of normal liver, hepatocellular cancer, liver metastases of pancreatic cancer, normal pancreas and primary pancreatic cancer using the mouse version of this antibody. Furthermore, the cytotoxic properties of this antibody were determined in a resazurin viability assay on HUVECs and two different tumour cell lines (Wang et al, 2019; pmid：31398471). To evaluate which ligands on the neutrophil mediate adhesion to VCAM-1, flow cytometry was performed on human neutrophils using the mouse version of this antibody (Lomakina and Waugh, 2009; pmid：19134480). To determine the interaction of cells with titanium, immunofluorescence was used on human osteoblastic cells with the mouse version of this antibody. Furthermore, this antibody was used for flow cytometry on MG-63 cells (Lüthen et al, 2005; pmid：15585246). To study how megakaryocytes differentiate and release platelets in the blood, immunofluorescence was performed on megakaryocytes using the mouse version of this antibody (Malara et al, 2011; pmid：21131589). To determine the regulatory effect of interleukin-4 (IL-4) on the expression and function of lymphocyte adhesion receptors involved in IL-2-induced cell aggregation, flow cytometry on peripheral blood lymphocytes using the mouse version of this antibody was performed.Furthermore, peripheral blood lymphocytes were used for immunofluorescence using this antibody (Vyth- Dreese et al, 1993; pmid：8473014).

Vyth-Dreese et al. Regulatory effect of interleukin-4 (IL-4) on the expression and function of lymphocyte adhesion receptors involved in IL-2-induced cell aggregation Immunology. 1993 Feb;78(2)：244-51. PMID：8473014

Human recombinant interleukin-4 (rIL-4) was studied for its capacity to inhibit rIL-2- induced lymphoid cell aggregation. In contrast to rIL-2, rIL-4 was unable to induce cluster formation by itself. However, when added simultaneously with rIL-2 to cultures of freshly isolated peripheral blood lymphocytes (PBL), rIL-4 inhibited cell aggregation in a dose-dependent way.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.