Cat. No.
MABL-947
Application
Activate, IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
G28.5
From
Recombinant Antibody
Specificity
48 kD Type I glycoprotein, a member of the TNFR superfamily primarily expressed on normal or malignant B cells or B cell lines, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells.
Alternative Names
TNFRS5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor CDw40; CD154R
UniProt
P25942
Immunogen
Human erythrocyte-rosette-negative tonsillar lymphocytes.
Application Notes
This antibody detects CD40 by immunostaining. The original mouse IgG1 format was shown to stimulate B-cells (Bishop 2012 and references therein).
Antibody First Published
Clark EA, Ledbetter JA. Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4494-8. PMID:3487090
Note on publication
Describes the making of the antibody, shows it recognised a 50-kDa polypeptide expressed on all B cells using immunoprecipitation and flow cytometry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
