Cat. No.
MABL-938
Application
agonist, IP, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
1C10
From
Recombinant Antibody
Specificity
This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes.
Alternative Names
TNFRSF5; B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; MGC9013; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
UniProt
P27512
Immunogen
The original antibody was generated by immunizing a rat with 20 µg of soluble extracellular domain of recombinant murine CD40 (sCD40).
Application Notes
This antibody was reported to directly induce a specific proliferative response from mature murine B cells, up-regulate several B cell surface antigens, and rescue immature B lymphoma cells from anti-IgM-induced growth arrest. This initial screening of hybridoma supernatants for reactivity towards CD40 transfected L cells was done using flow cytometry. This antibody recognized recombinant CD40 expressed on L cells and the soluble extracellular domain of murine CD40 coated onto microtiter plates in an ELISA. This antibody was also used in the immunofluorescent staining of adult murine spleen cells. The staining was restricted to the B220+ population. This antibody also specifically immunoprecipitated a 45- kDa protein from the surface of purified murine splenic B cells (PMID: 7519997). This antibody was capable of blocking binding of FGK45 to CD40 on murine B cells in vitro (PMID: 24416732). The mouse IgG1 version of this antibody had a tenfold higher affinity for mouse FcγRIIB as compared to mouse IgG2a or the parental rat IgG2b (PMID: 21852502). The mouse IgG1 version of this antibody was used in the treatment of M38 tumor bearing mice and it was reported that there was a strong increase in PD-L1 expression both on tumor- infiltrating monocytes and TAMs (PMID: 25623164).
Antibody First Published
Heath et al. Monoclonal antibodies to murine CD40 define two distinct functional epitopes. Eur J Immunol. 1994 Aug;24(8):1828-34. PMID:7519997
Note on publication
This paper discusses the generation and functional characterization of two antibodies that recognize two distinct CD40 epitopes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
