Cat. No.
MABL-937
Application
Blocking, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10C12
From
Recombinant Antibody
Specificity
This antibody recognises LSEKKTCQCPHRFQKTCSPI peptide of human CD4 epitope within amino acids 414 and 433 of the cytoplasmic tail region. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus.The cytoplasmic tail region transmits a signal in response to p56-lck. HIV nef is also known to interact with the cytoplasmic tail of CD4.
Alternative Names
Leu3/T4; 024; CD4 cytoplasmic tail; T-cell surface glycoprotein CD4; T- cell surface antigen T4/Leu-3
UniProt
P01730
Immunogen
This antibody was raised against a multiple antigenic peptide; a tetramer linked through a lysine backbone.
Application Notes
10C12 antibody reacts with a poorely studied tail region of human CD4 and might be used to conduct reasearch on the role of this fragment of the protein. Furthermore, this antibody can be used for studies on expression of CD4. One group utilised 10C12to perform Western blot analysis of CD4 expression in cerebellar granule neurons when researching interleukin-16 induced c-Fos expression (Fenster et al., 2010). This antibody may be also used as an effective blocking agent inhibiting Il-16 (Atanackovic et al., 2012). Finally, 10C12 has also been recommended for studies of the role of selenium metabolism and selenoproteins in diseases, such as infectious diseases (HIV) and cancer (Ware, 2003).
Antibody First Published
Ware Gene regulation and overlooked coding regions : the case for translation past the 3'-UGA codon in CD4. Ph.D. Thesis. University of Georgia, College of Pharmacy.(2003)
Note on publication
This thesis describes the generation and characterisation of TL2.1 antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
