Cat. No.
MABL-917
Application
inhibition, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb2
From
Recombinant Antibody
Specificity
This antibody is specific for human CD39, also known as NTPDase 1 (ectonucleoside triphosphate diphosphohydrolase) or vascular CD39. CD39 works in concert with CD73 (ecto-5'- nucleotidase) to hydrolyze extracellular adenosine triphosphate (ATP) and adenosine diphosphate (ADP), generating adenosine. The resulting adenosine binds to adenosine receptors, inhibiting T-cell and natural killer (NK)-cell responses, thus suppressing immune system activity. Additionally, CD39 may play a role in preventing platelet aggregation by converting platelet-activating ADP into AMP.
Alternative Names
ENTPD1; NTPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen
UniProt
P49961
Immunogen
The original antibody was generated by humanizing an anti-CD39 murine antibody clone.
Application Notes
The specificity of the original version of this antibody (Human IgG1, Fc Silent) was assessed using ELISA. Among the tested targets — vascular CD39 and the CD39 isoforms L1, L2, L3, and L4 — the antibody exclusively bound to vascular CD39. In a flow cytometry (FC) assay using Ramos human lymphoma cells, which express CD39, the antibody demonstrated strong binding. Additionally, the antibody exhibited potent inhibition of CD39 activity, as measured by a MALDI-TOF (Mass Spectrometry) assay, effectively blocking over 90% of CD39's enzymatic activity (US20220056145A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
