Cat. No.
MABL-889
Application
WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2E 10
From
Recombinant Antibody
Specificity
The antibody is specific to a class III epitope on CD34, which is not susceptible to cleavage by neuraminidase, chymopapain or glycoprotease.
Alternative Names
Hematopoietic progenitor antigen; Hematopoietic progenitor cell antigen CD34; 2E 10
UniProt
P28906
Immunogen
The antibody was raised by repeatedly immunising BALB/c mice with KG-1a cells.
Application Notes
This antibody, labelled with fluoroscein isothiocyanate, has been used in flow cytometry to assess the loss of expression of CD34 epitopes following enzyme incubation (Qian et al., 2008). The antibody has been used as a primary antibody against CD34 in Western blot analysis, using HRP- conjugated goat anti-mouse IgG as a secondary antibody.
Antibody First Published
Qian et al. Development of new versions of anti-human CD34 monoclonal antibodies with potentially reduced immunogenicity Biochem Biophys Res Commun. 2008 Mar 7;367(2):497-502. PMID：18190781
Note on publication
Describes the original generation of this antibody and the construction of chimeric antibodies with human IgG constant regions.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
