Cat. No.
MABL-835
Application
Block, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey, Mouse
Clone No.
5B7
From
Recombinant Antibody
Specificity
This antibody binds human BAFF protein and cross reacts with mouse and cynomolgus monkey BAFF protein. B-cell-activating factor (BAFF/CD257) is a cytokine belonging to the tumour-necrosis factor (TNF) family. This cytokine binds to TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF is a survival factor for B cells that controls B-cell maturation. It is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.
Alternative Names
BLyS; TNFSF13B; TALL-1; TNFSF20; ZTNF4; Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; B-cell-activating factor; Dendritic cell-derived TNF-like molecule; TNF and APOL-related leukocyte expressed ligand 1
UniProt
Q9Y275
Immunogen
The original antibody was isolated from a naïve human scFv phage library using multiple panning strategies including solid-phase panning with immobilized CD47 and solution-phase panning using biotinylated CD47.
Application Notes
This antibody is capable of reacting with both soluble and membrane bound human, mouse and cynomolgus monkey BAFF/ BLyS protein. The binding ability of this antibody to membrane bound CHO-human BLys, HEK293-mouse BLyS and CHO-cynomolgus monkey BLyS protein was determined using flow cytometry. This antibody is capable of blocking the interaction between human BAFF and BR3.This antibody was also capable of neutralizing BLyS-mediated B-cell proliferation. The binding affinity characterization of this antibody was done using SPR. It was reported that the original IgG antibody binds human and mouse BLyS with a binding affinity of Kd= 4X10^(-10) M and Kd= 1.45X10^(-11) M respectively (US20170101466).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
