Cat. No.
MABL-825
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
M1/69
From
Recombinant Antibody
Specificity
This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis.
Alternative Names
Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen
UniProt
P24807
Immunogen
The original antibody was generated by immunizing DA rats with B10 mouse splenic T cells and concanavalin A-activated splenocytes.
Application Notes
This antibody was capable of recognizing CD24 antigen from microsomal membrane preparation of different mouse cells through western blot (PMID: 1696896). This antibody is recommended for immunophenotypic analysis of CD24 expressed on mouse B and T cells using flow cytometry (PMID: 17702899; 20133615; 20203098; 33111080). This antibody (dilution 1：100) was also used in the immunohistochemical analysis of CD24 expression in mouse thyroid cells using 5-μm zinc-fixed, paraffin- embedded thyroid sections (PMID： 18801910). This antibody was also reported to be used in the flow cytometric and immunohistochemical analysis of murine CD24 expressed on the surface of normal human breast epithelial cells early after their infection with a retroviral vector (PMID： 9232274). This antibody was also used in the immunofluorescence staining of murine spinal cord sections (PMID： 17475850).
Antibody First Published
Springer et al. Monoclonal xenogeneic antibodies to murine cell surface antigens： identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8)：539-51. PMID：81133
Note on publication
Describes the generation and characterization of different antibodies against mouse cell surface antigens.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
