Cat. No.
MABL-824
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
G7
From
Recombinant Antibody
Specificity
This antibody binds human CD24.
Alternative Names
Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen
UniProt
P25063
Immunogen
The original antibody was generated by immunizing BALB/c mice with a CD24 core region polypeptide and hemocyanin KLH conjugate (CD24-KLH) as immunogen.
Application Notes
This antibody showed specific binding affinity to recombinant human CD24 but not to unrelated recombinant Fc in a western blot. The binding specifity of G7 towards CD24 was also confirmed with competitive ELISA. Flow cytometric assay was carried out to assess the affinities of G7 to native CD24 expressed on Huh7 and HT29 cells with HCT116 cells as control. G7mAb demonstrated significant binding to both Huh7 and HT29 cells with rates 52.9% and 41.3% respectively. This antibody was also used in immunohistochemical staining of tumor samples from Huh7 xenograft nude mice (PMID: 26255089).
Antibody First Published
He et al. A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging. Immunobiology. (2015); 220(12):1328-36. PMID:26255089
Note on publication
Describes the generation of an scFv version of this antibody and tests its use in targeted tumor therapy and diagnostic potentials in vitro and in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
