Cat. No.
MABL-819
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
5E 3
From
Recombinant Antibody
Specificity
This antibody is specific for human CD23. CD23 is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B- cells.
Alternative Names
Low affinity immunoglobulin epsilon Fc receptor; FCER2; BLAST-2; C- type lectin domain family 4 member J; Fc-epsilon-RII; Immunoglobulin E-binding factor; Lymphocyte IgE receptor
UniProt
P06734
Immunogen
The mouse version of this antibody was raised by immunizing a BALB/c mouse with Daudi (human Burkitt's lymphoma cell-line).
Application Notes
To examine B1 and B2 cells for their ability to be rendered unresponsive by using an anti-lg tolerance protocol that is independent of receptor specificity, flow cytometry was performed on mouse splenic B cell suspensions using the mouse version of this antibody (Liou et al, 1995; pmid:7759864).
Antibody First Published
Liou et al. Differential susceptibility to tolerance induction in vitro of splenic B cells from several transgenic mouse lines. Role of B1 cells J Immunol. 1995 Jun 15;154(12):6262-74.PMID:7759864
Note on publication
Spleen cells from transgenic mice, whose rearranged Ig receptors reflect the repertoires of B1 (CD5+ and "sister" B cells) or normal B2 cells, were examined for their ability to be rendered unresponsive. By using an anti-Ig tolerance protocol that is independent of receptor specificity, we previously reported that peritoneal B cells, containing primarily CD5+ and "sister" B cells, were not susceptible to unresponsiveness. Herein, we show that splenic B cells from two separate transgenic mouse lines, each expressing a B1-type receptor, are resistant to tolerance induction in vitro.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
