Cat. No.
MABL-809
Application
in vivo, inhibition, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
MB22-11
From
Recombinant Antibody
Specificity
This antibody is specific for mouse CD22.
Alternative Names
CD22; BL-CAM; B-cell receptor CD22; Siglec-2; T-cell surface antigen Leu- 14; Sialic acid-binding Ig-like lectin 2; B-lymphocyte cell adhesion molecule
UniProt
P35329
Immunogen
The original antibody was raised by immunizing a CD22-/- mice with mouse CD22.
Application Notes
The ability of the original antibody to deplete B cell in vivo in mice was tested. It showed that is was able to deplete B cells NZB/W F1 and C57BL/6 mice. This antibody was used for an ELISA (Haas et al., 2010; PMID
Antibody First Published
Haas et al. CD22 ligand binding regulates normal and malignant B lymphocyte survival in vivo J Immunol. 2006 Sep 1;177(5)：3063-73. PMID：16920943
Note on publication
In the original paper mouse anti-mouse CD22 mAbs were generated that inhibit CD22 ligand binding to varying degrees, to understand how CD22 ligand interactions affect B cell function in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
