Cat. No.
MABL-808
Application
RIA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LL2 (EPB2)
From
Recombinant Antibody
Specificity
This antibody recognises CD22, which is a cytoplasmic molecule expressed in progenitor and early-pre B cells.
Alternative Names
Leu14; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL- CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; EPB2; EPB-2
UniProt
P20273
Immunogen
This antibody was raised by immunising Balb/c mice with Raji cells or a fragmented cell mixture.
Application Notes
This antibody has been used in research on the diagnosis and treatment of non- Hodgkin's B cell lymphoma. Specificity to B-cell line of the LL2 (EPB-2) antibody was confirmed by radioimmunobinding and flow cytometry, where it did not bind to any other targets, such as T cells and normal diploid cells at the same time labelling malignant lymphoma tissue (Pawlak-Byczkowska et al., 1989).
Antibody First Published
Pawlak-Byczkowska et al. Two new monoclonal antibodies, EPB-1 and EPB- 2, reactive with human lymphoma. Cancer Res. 1989 Aug 15;49(16)：4568-77. PMID：2663143
Note on publication
This article describes the generation and characterisation of the LL2 (EPB-2) antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
