Cat. No.
MABL-805
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
THB-5
From
Recombinant Antibody
Specificity
This antibody is specific for human CD21 (complement receptor type 2), which is expressed on B-cells and participates in B cell activation. It recognises a distinct antigenic site to the anti-B2 antibody (Tedder et al, 1984).
Alternative Names
CR2; Complement receptor type 2; ; complement C3d receptor;C3dR; C3DR; Epstein-Barr virus receptor; EBV receptor; cluster of differentiation 21; THB 5
UniProt
P20023
Immunogen
This antibody was raised by immunising BALB/c mice with human B cells of the SB line. Spleen cells were then removed and fused with Ag8.653 myeloma cells, to produce stable hybridomas.
Application Notes
The binding specificity of this antibody has been confirmed in flow cytometry and immunofluorescence analysis (Tedder et al, 1984), with only B cell reactivity observed. Though this antibody binds to CD21, which acts as a receptor for Epstein-Barr virus (EBV), it cannot alone block viral entry into B cells (Fingeroth et al, 1984). EBV virions engineered to express this antibody at their surface have been used to probe the need for CD21 binding to achieve viral entry (Busse et al, 2010).
Antibody First Published
Tedder et al. Expression of C3d receptors during human B cell differentiation： immunofluorescence analysis with the HB-5 monoclonal antibody Journal of immunology,1984, 133(2)： 678-83 PMID：6234356
Note on publication
Describes the original generation of this antibody, and its use in FC and IF analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
