Cat. No.
MABL-791
Application
functional assay, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
VET309 (CD20-6)
From
Recombinant Antibody
Specificity
This antibody binds canine (dog) CD20 and does not cross react with cat CD20. This protein plays an important role in the regulation of cellular calcium influx necessary for development, differentiation, and activation of B-lymphocytes.
Alternative Names
MS4A1; B-lymphocyte antigen CD20; Membrane-spanning 4-domains subfamily A member 1
UniProt
Q3C2E2
Immunogen
The original antibody was generated by immunizing mice with CHO-DG44 cells expressing canine CD20.
Application Notes
The binding reactivity of this antibody to recombinant dog CD20 expressed on the surface of CHO cells was determined using flow cytometry. This antibody binds recombinant dog CD20 with a binding affinity of Kd= 63.41 nM. This antibody exhibited a strong staining of B cells in a immunohistochemical staining of lymph node tissues collected from dogs with B-cell lymphoma. The ability of this antibody to alter the proliferation of lymphoma cells was checked using cell assays. This antibody was reported to cause a 27% reduction in proliferation of lymphoma cells (US9616120).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
