Cat. No.
MABL-787
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Human
Clone No.
NCD1.2 (scFv-3)
From
Recombinant Antibody
Specificity
This antibody is specific for the amino acid sequence 'PSEKNSLSI' of dog CD20. It also cross reacts with human CD20. CD20 is a 33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre B-cells. This B-lymphocyte-specific membrane protein plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. It also functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
Alternative Names
Bp35; Leu-16; MS4A1; Leukocyte surface antigen Leu-16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Membrane-spanning 4-domains subfamily A member 1
UniProt
Q3C2E2; P11836
Immunogen
The original antibody was generated by immunizing 5–8 weeks old BALB/c mice with a canine CD20 peptide (NCDPANPSEKNSLSIQYCGS) at a final concentration of 100 μg/ml in complete Freud’s adjuvant.
Application Notes
The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID： 26894679)
Antibody First Published
Jain et al. The Development of a Recombinant scFv Monoclonal Antibody Targeting Canine CD20 for Use in Comparative Medicine. PLoS One. 2016; 11(2)： e0148366. PMID：26894679
Note on publication
Describes the development and characterization of a recombinant anti-canine CD20 scFv antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
