Cat. No.
MABL-783
Application
radioimmunotherapy, WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
B9E9
From
Recombinant Antibody
Specificity
This antibody is specific for CD20, which is expressed by normal and malignant B cells. It does not cross-react with murine CD20.
Alternative Names
MS4A1; B-lymphocyte antigen CD20;MS4A1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437
UniProt
P11836
Immunogen
This antibody was raised against human CD20.
Application Notes
This antibody has been used in western blot detection of CD20 in human plasma, revealing elevated CD20 levels in plasma from chronic lymphocytic leukemia patients (Manshouri et al, 2003). An scFv derived from this antibody fused to streptavidin has been investigated as a method of pretargeted radioimmunotherapy for lymphomas (Schultz et al, 2000), and has been demonstrated to be non-toxic in a phase I trial of non-Hodgkin lymphoma patients (Forero et al, 2004). Additionally, measles viruses engineered to express this scFv are able to enter CD20+ target cells through interaction with CD20 antigen, with clinical relevance for lymphoma therapy (Bucheit et al, 2003).
Antibody First Published
Schultz et al. A Tetravalent Single-chain Antibody-Streptavidin Fusion Protein for Pretargeted Lymphoma Therapy Cancer Res. 60 (23), 6663-6669 (2000) PMID：11118050
Note on publication
Describes the generation of an scFv from this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
