Cat. No.
MABL-782
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ABM58020-32A8-PU
From
Recombinant Antibody
Specificity
This antibody binds human CD20. CD20 is a 33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre B-cells. This B-lymphocyte-specific membrane protein plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. It also functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.
Alternative Names
Bp35; Leu-16; MS4A1; Leukocyte surface antigen Leu-16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Membrane-spanning 4-domains subfamily A member 1; L26
UniProt
P11836
Immunogen
The original antibody was generated by immunizing BALB/c mice with CD20-KLH conjugate in Freund's complete adjuvant.
Application Notes
This binding specificity of this antibody for human CD20 was checked using ELISA. This antibody positively binds human CD20-BSA in an ELISA. The affinity constant of the original IgG2b antibody was determined to be 3.07 × 10^10 M^(-1). This antibody can recognize human CD20 in an immunoblot assay. This antibody is similar to commercially available anti-CD20 antibody L26 in immunohistochemical staining of B cell lymphomas. This antibody was used in the immunohistochemical staining of B cell lymphomas, T cell lymphomas, tonsil tissue and appendectonic tissues (CN111363043).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
