Cat. No.
MABL-780
Application
IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Baboon, chimpanzee, gibbon, langur, Marmoset, owl monkey, Human
Clone No.
2H7
From
Recombinant Antibody
Specificity
This antibody is specific for CD20, which is expressed by normal and malignant B cells.
Alternative Names
MS4A1; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437
UniProt
P11836
Immunogen
This antibody was raised by hyperimmunising a C57BL/6 mouse with the Ia- B cell line 6.16 cl. 3.
Application Notes
This antibody has been used in flow cytometry and quantitative immunofluorescence analysis of human peripheral blood mononuclear leukocytes (Yanase et al, 1986).It has also been used to precipitate CD20 from lysates of the Raji human B cell line (Deans et al, 1995). This antibody has been exploited as part of a an avidin-biotin immunoadsorption system to eliminate Daudi lymphoblasts from human bone marrow (Berenson et al, 1986). A mouse-human chimeric version of this antibody effectively mediates antibody-dependent cellular cytotoxicity with human effector cells and complement-dependent cytotoxicity with human complement (Liu et al, 1987).
Antibody First Published
Ledbetter et al. Surface phenotype and function of tonsillar germinal center and mantle zone B cell subsets Human Immunology, 1986, Vol.15(1), pp.30-43 PMID：2419284
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
