Cat. No.
MABL-779
Application
depleting, stimulatory, therapeutic
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1F5
From
Recombinant Antibody
Specificity
This antibody is specific for CD20, which is expressed by normal and malignant B cells.
Alternative Names
MS4A1; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437
UniProt
P11836
Immunogen
This antibody was raised by hyperimmunising BALB/c mice with the Raji Burkett's lymphoma line and the NALM-6 pre B leukaemia line.
Application Notes
This antibody, either alone or in combination with T cell derived factors, induces B cell proliferation via CD20 cross-linking (Clark et al, 1985).When administered to patients with refractory malignant B cell lymphomas, this antibody depletes circulating and lymph node tumour cells, with minimal toxicity (Press et al, 1987). A murine/human chimeric IgG1 construct of this antibody has been shown to lyse Burkitt's lymphoma (5RAJI) cells (Stockmeyer et al, 2000), while IgG1 and IgA2 isotypes of this antibody have been shown to kill B cells in vitro and in deplete B cells in vivo (Lohse et al, 2018). The humanized version of this antibody can be provided upon request.
Antibody First Published
Ledbetter et al. Surface phenotype and function of tonsillar germinal center and mantle zone B cell subsets Human Immunology, 1986, Vol.15(1), pp.30-43 PMID：2419284
Note on publication
Describes the original generation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
