Cat. No.
MABL-753
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
YFC51.1
From
Recombinant Antibody
Specificity
This antibody binds human CD18 and recognizes amino acid residues between 122 and 344, more particularly amino acids from two regions 123–163 and 302–344 of the human CD18 molecule (PMID: 10779511). Binding of CD18 and CD11 results in the formation of Lymphocyte Functions Associated Antigen 1 (LFA-1), a protein found on B cells, all T cells, macrophages, neutrophils and NK cells. LFA-1 is involved in adhesion and binding to antigen presenting cells through interactions with the surface protein ICAM-1.
Alternative Names
ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA- 1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins
UniProt
P05107
Immunogen
The original antibody was generated by immunizing DA rats with human neutrophils.
Application Notes
The original rat IgG2b version of this antibody was successful in blocking CD18. The human IgG1 version of this antibody was able to block CD18 but unable to bind human Clq and did not mediate antibody-dependent cellular cytotoxicity (PMID: 7688398). This antibody was used as one of the antibodies to study the Aeffect of anti-LFA-1 panel of abs on T cell adhesion to ICAM-1 and ICAM-3 using ELISA (PMID: 7518468).
Antibody First Published
Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-I family, and leukocyte common antigens: new and previously defined clusters. Leukocyte Typing Ill. White Cell Differentiation Antigens. A. J. McMichael, editor. Oxford University Press, Oxford. 784-803. ISBN 10: 0192615521 / ISBN 13:9780192615527
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
