Cat. No.
MABL-748
Application
SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
OX-130
From
Recombinant Antibody
Specificity
This antibody recognizes both common alleles of the human activating SIRPβ1 receptor, does not recognize SIRPγ, but cross-reacts with one of the common alleles of the inhibitory human SIRPα receptor.
Alternative Names
SIRP-beta-1; Signal-regulatory protein beta-1; CD172 antigen-like family member B
UniProt
O00241
Immunogen
Recombinant SIRPβ1 was purified by nickel affinity chromatography and used to immunize mice.
Application Notes
This antibody binds both common alleles of the human activating SIRPβ1 receptor, but ELISA and surface plasmon resonance results have shown that it reacts with one of the two common alleles of SIRPα, potentially yielding different outcomes when this antibody is used in assays from different individuals.
Antibody First Published
Hatherley et al. OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele Monoclon Antib Immunodiagn Immunother. 2016 Feb 1; 35(1)： 57–59. PMID：26871367
Note on publication
Describes the original production of monoclonal antibody OX130, and the characterisation of its specificity using ELISA and SPR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
