Cat. No.
MABL-747
Application
functional, IP, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-41
From
Recombinant Antibody
Specificity
This antibody binds to rat SIRP alpha, which is selectively expressed by myeloid and neuronal cells.
Alternative Names
SIRPA; BIT; MFR; MYD-1; MYD1; P84; PTPNS1; SHPS1; SIRP; Signal- regulatory protein alpha; signal regulatory protein alpha; OX41; MRC OX-41; MRC OX 41
UniProt
P97710
Immunogen
Rat resident peritoneal cells injected into BALB/c mice.
Application Notes
This antibody binds to SIRP alpha, and can consequently be used to label neuronal cells and myeloid cells, namely macrophages, monocytes, granulocutes and dendritic cells, in immunofluorescence and immunohistochemical studies (Robinson, 1986). Anti-SIRP ligation induces nitric oxide synthesis and oxidative burst in macrophages (Adams, 1998).
Antibody First Published
Robinson et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.Immunology 1986 57 239-247 PMID：3512425
Note on publication
Describes the original use of this antibody to detect SIRP alpha in labelling, immunoperoxidase and immunofluorescence studies to investigate macrophage heterogeneity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
