Cat. No.
MABL-724
Application
RIA, WB, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-47
From
Recombinant Antibody
Specificity
This antibody is specific for rat CD147, which has a broad distribution; OX-47 labels T and B cell blasts, peritoneal macrophages, the endothelium of all except large vessels and kidney tubules, but does not label TDL and thymocytes.
Alternative Names
Basigin; BSG; extracellular matrix metalloproteinase inducer; EMMPRIN; Glycoprotein CE9; OX-47 antigen
UniProt
P26453
Immunogen
Mice immunized with rat T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Application Notes
This antibody binds mouse CD147, and has been used in FC and IHC-fr analysis to characterise CD147 and detect the cell types on which it is expressed, indicating that CD147 is a lymphocyte activation marker (Paterson, 1987). This antibody failed to give immunoprecipitates from radiolabelled activated T cells.
Antibody First Published
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 M r detected only on CD4 positive T blasts Molecular Immunology, 1987, Vol.24(12), pp.1281- 1290 PMID；2828930
Note on publication
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
